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產品詳情
  • 產品名稱:重組人類4-1BB受體/ TNFRSF9蛋白

  • 產品型號:rHu4-1BB Receptor/TNFRSF9
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人類4-1BB受體/ TNFRSF9蛋白與其它公司提供的重組蛋白不同,rHu4-1BB Receptor/TNFRSF9蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類4-1BB受體/ TNFRSF9蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人類4-1BB受體/ TNFRSF9蛋白

Synonyms TNFRSF9, CD137 Antigen, T-cell Antigen ILA
Species Human
Accession Q07011
GeneID 3604
Source Escherichia coli.
Molecular Weight 重組人類4-1BB受體/ TNFRSF9蛋白Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence ERTRSLQDPC SNCPAGTFCD NNRNQICSPC PPNSFSSAGG QRTCDICRQC KGVFRTRKEC SSTSNAECDC TPGFHCLGAG CSMCEQDCKQ GQELTKKGCK DCCFGTFNDQ KRGICRPWTN CSLDGKSVLV NGTKERDVVC GPSPADLSPG ASSVTPPAPA REPGHS
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組人類4-1BB受體/ TNFRSF9蛋白Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inibition was seen using a concentration of 1 μg for both 4-1BB Ligand and 4-1BB Receptor.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 8.0, 150 mM NaCl.
Endotoxin 重組人類4-1BB受體/ TNFRSF9蛋白Less than 1 EU/μg of rHu4-1BB Receptor as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類4-1BB受體/ TNFRSF9蛋白
SDS-PAGE
Reference 1. Arch RHandThompson CB. 1998. Mol Cell Biol, 18: 558-65.
2. Barao I. 2012. Front Immunol, 3: 402.
3. Taraban VY, Rowley TF, O'Brien L, et al. 2002. Eur J Immunol, 32: 3617-27.
4. Marin V, Kakuda H, Dander E, et al. 2007. Exp Hematol, 35: 1388-97.
Background 4-1BB receptor, also named TNFRSF9 is a member of the TNF superfamily of receptors. It is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB receptor binds to 4-1BBL, and they co-stimulate activity for activated T cells. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. Crosslinking of 4-1BB Receptor enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

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